Composite

Part:BBa_K1604022:Design

Designed by: Veronica Pinamonti   Group: iGEM15_UNITN-Trento   (2015-08-20)


araC-pBAD + β-carotene + J23100 + blh


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 9
    Illegal NheI site found at 32
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2041
    Illegal BamHI site found at 4082
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 247
    Illegal NgoMIV site found at 3618
    Illegal NgoMIV site found at 3748
    Illegal AgeI site found at 1876
    Illegal AgeI site found at 2833
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1858


Design Notes

The two parts was joined together by strandard assembly, before it beta-carotene was amplified by Phusion PCR.

NOTE This device was built using a part from the Cambridge 2009 team . Be careful when operating this part, because the SpEI site in the suffix is missing. The part from Cambridge 2009 had this mistake and so the error was introduced in our part. We realized that after sequencing. Other than that the device works well.

Source

AraC-pBAD promoter comes from E.coli genome, while ctrEIBY are from Pantoea ananatis (formerly Erwinia uredovora) genome. Blh gene and its promoter are synthetized and codon optimezed by GeneScript.

References